First Report of Pineapple Heart Rot Caused by Phytophthora nicotianae in Hainan Province, China.
Identifieur interne : 001314 ( Main/Exploration ); précédent : 001313; suivant : 001315First Report of Pineapple Heart Rot Caused by Phytophthora nicotianae in Hainan Province, China.
Auteurs : H F Shen [République populaire de Chine] ; B R Lin [République populaire de Chine] ; J X Zhan [République populaire de Chine] ; X M Pu [République populaire de Chine]Source :
- Plant disease [ 0191-2917 ] ; 2013.
Abstract
Pineapple (Ananas comosus) is an economically important tropical fruit in Hainan Province, China. During September to November 2011, heart rot disese of pineapple was found in Ledong and Wangning of Hainan Province. A survey of 150 ha producing areas of pineapple revealed that the fields were affected at an incidence ranging from 25% to 30%. Infected plants showed water-soaked lesions and soft rot on the base of heart leaves near the soil surface. Heart leaves of infected plants were easily pulled out. As the disease progressed, plants collapsed and died. Diseased tissue fragments (2 × 2 mm) were surface-disinfected for 10 min with 0.3% NaClO, then rinsed three times in sterile water, and plated to 10% V8 juice agar (4). Inoculated dishes were incubated at 26°C in the dark. After 5 days, Phytophthora (identified by the presence of coenocytic hyphae and papillate sporangia) were isolated from the tissue cultures, which has aseptate hyphae. Sporangia were papillate, noncaducous, oval or spherical, and 34.5 to 58.2 μm. Clamydospores, both terminal and intercalary, were also spherical, and were 23.4 to 34.0 μm (2). The ITS region of rDNA was amplified using primers ITS4/ITS5, and the 927-bp product of the ITS showed 99% sequence identity to Phytophthora nicotianae (GenBank Accession No. JF792540), and the sequence was accessed to NCBI (JX978446). Pathogenicity tests were confirmed by irrigating the wounded stem bases of 10 2-month-old pineapple plants with 50 ml of P. nicotionae zoospore solution (15,000 zoospores/ml), and another 10 plants of the same cultivar inoculated with sterile water served as controls. Plants were placed in pots in a greenhouse at 28°C and 90% relative humidity. After 9 days, soft rot was observed clearly on the base of heart leaves of all 10 inoculated plants, while the control plants appeared normal. P. nicotianae was reisolated from the infected pineapple plants, and confirmed to be the same as the inoculated pathogen by conducting a ITS rDNA sequence comparison and morphological characteristics. P. nicotianae was previously reported as the causal agent of heart rot of pineapple in Hawaii, U.S.A. (3) and Guangdong Province of China (1). To our knowledge, this is the first report of P. nicotianae on pineapple in Hainan Province, China. References: (1) J. Z. Chen et al. J. Yunnan Agric. Univ. 8:134, 2003. (2) H. H. Ho. Mycologia 73:705, 1981.(3) K. W. Howard et al. Plant Dis. Rep. 48:848, 1964. (4) X. B. Zheng. Page 81 in: Phytophthora and its Research Technology. China Agricultural Press, Beijing, 1997.
DOI: 10.1094/PDIS-11-12-1017-PDN
PubMed: 30722247
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">First Report of Pineapple Heart Rot Caused by Phytophthora nicotianae in Hainan Province, China.</title><author><name sortKey="Shen, H F" sort="Shen, H F" uniqKey="Shen H" first="H F" last="Shen">H F Shen</name><affiliation wicri:level="1"><nlm:affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Lin, B R" sort="Lin, B R" uniqKey="Lin B" first="B R" last="Lin">B R Lin</name><affiliation wicri:level="1"><nlm:affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhan, J X" sort="Zhan, J X" uniqKey="Zhan J" first="J X" last="Zhan">J X Zhan</name><affiliation wicri:level="1"><nlm:affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Pu, X M" sort="Pu, X M" uniqKey="Pu X" first="X M" last="Pu">X M Pu</name><affiliation wicri:level="1"><nlm:affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:30722247</idno><idno type="pmid">30722247</idno><idno type="doi">10.1094/PDIS-11-12-1017-PDN</idno><idno type="wicri:Area/Main/Corpus">001309</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001309</idno><idno type="wicri:Area/Main/Curation">001309</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001309</idno><idno type="wicri:Area/Main/Exploration">001309</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">First Report of Pineapple Heart Rot Caused by Phytophthora nicotianae in Hainan Province, China.</title><author><name sortKey="Shen, H F" sort="Shen, H F" uniqKey="Shen H" first="H F" last="Shen">H F Shen</name><affiliation wicri:level="1"><nlm:affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Lin, B R" sort="Lin, B R" uniqKey="Lin B" first="B R" last="Lin">B R Lin</name><affiliation wicri:level="1"><nlm:affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhan, J X" sort="Zhan, J X" uniqKey="Zhan J" first="J X" last="Zhan">J X Zhan</name><affiliation wicri:level="1"><nlm:affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Pu, X M" sort="Pu, X M" uniqKey="Pu X" first="X M" last="Pu">X M Pu</name><affiliation wicri:level="1"><nlm:affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pineapple (Ananas comosus) is an economically important tropical fruit in Hainan Province, China. During September to November 2011, heart rot disese of pineapple was found in Ledong and Wangning of Hainan Province. A survey of 150 ha producing areas of pineapple revealed that the fields were affected at an incidence ranging from 25% to 30%. Infected plants showed water-soaked lesions and soft rot on the base of heart leaves near the soil surface. Heart leaves of infected plants were easily pulled out. As the disease progressed, plants collapsed and died. Diseased tissue fragments (2 × 2 mm) were surface-disinfected for 10 min with 0.3% NaClO, then rinsed three times in sterile water, and plated to 10% V8 juice agar (4). Inoculated dishes were incubated at 26°C in the dark. After 5 days, Phytophthora (identified by the presence of coenocytic hyphae and papillate sporangia) were isolated from the tissue cultures, which has aseptate hyphae. Sporangia were papillate, noncaducous, oval or spherical, and 34.5 to 58.2 μm. Clamydospores, both terminal and intercalary, were also spherical, and were 23.4 to 34.0 μm (2). The ITS region of rDNA was amplified using primers ITS4/ITS5, and the 927-bp product of the ITS showed 99% sequence identity to Phytophthora nicotianae (GenBank Accession No. JF792540), and the sequence was accessed to NCBI (JX978446). Pathogenicity tests were confirmed by irrigating the wounded stem bases of 10 2-month-old pineapple plants with 50 ml of P. nicotionae zoospore solution (15,000 zoospores/ml), and another 10 plants of the same cultivar inoculated with sterile water served as controls. Plants were placed in pots in a greenhouse at 28°C and 90% relative humidity. After 9 days, soft rot was observed clearly on the base of heart leaves of all 10 inoculated plants, while the control plants appeared normal. P. nicotianae was reisolated from the infected pineapple plants, and confirmed to be the same as the inoculated pathogen by conducting a ITS rDNA sequence comparison and morphological characteristics. P. nicotianae was previously reported as the causal agent of heart rot of pineapple in Hawaii, U.S.A. (3) and Guangdong Province of China (1). To our knowledge, this is the first report of P. nicotianae on pineapple in Hainan Province, China. References: (1) J. Z. Chen et al. J. Yunnan Agric. Univ. 8:134, 2003. (2) H. H. Ho. Mycologia 73:705, 1981.(3) K. W. Howard et al. Plant Dis. Rep. 48:848, 1964. (4) X. B. Zheng. Page 81 in: Phytophthora and its Research Technology. China Agricultural Press, Beijing, 1997.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30722247</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>97</Volume><Issue>4</Issue><PubDate><Year>2013</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>First Report of Pineapple Heart Rot Caused by Phytophthora nicotianae in Hainan Province, China.</ArticleTitle><Pagination><MedlinePgn>560</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-11-12-1017-PDN</ELocationID><Abstract><AbstractText>Pineapple (Ananas comosus) is an economically important tropical fruit in Hainan Province, China. During September to November 2011, heart rot disese of pineapple was found in Ledong and Wangning of Hainan Province. A survey of 150 ha producing areas of pineapple revealed that the fields were affected at an incidence ranging from 25% to 30%. Infected plants showed water-soaked lesions and soft rot on the base of heart leaves near the soil surface. Heart leaves of infected plants were easily pulled out. As the disease progressed, plants collapsed and died. Diseased tissue fragments (2 × 2 mm) were surface-disinfected for 10 min with 0.3% NaClO, then rinsed three times in sterile water, and plated to 10% V8 juice agar (4). Inoculated dishes were incubated at 26°C in the dark. After 5 days, Phytophthora (identified by the presence of coenocytic hyphae and papillate sporangia) were isolated from the tissue cultures, which has aseptate hyphae. Sporangia were papillate, noncaducous, oval or spherical, and 34.5 to 58.2 μm. Clamydospores, both terminal and intercalary, were also spherical, and were 23.4 to 34.0 μm (2). The ITS region of rDNA was amplified using primers ITS4/ITS5, and the 927-bp product of the ITS showed 99% sequence identity to Phytophthora nicotianae (GenBank Accession No. JF792540), and the sequence was accessed to NCBI (JX978446). Pathogenicity tests were confirmed by irrigating the wounded stem bases of 10 2-month-old pineapple plants with 50 ml of P. nicotionae zoospore solution (15,000 zoospores/ml), and another 10 plants of the same cultivar inoculated with sterile water served as controls. Plants were placed in pots in a greenhouse at 28°C and 90% relative humidity. After 9 days, soft rot was observed clearly on the base of heart leaves of all 10 inoculated plants, while the control plants appeared normal. P. nicotianae was reisolated from the infected pineapple plants, and confirmed to be the same as the inoculated pathogen by conducting a ITS rDNA sequence comparison and morphological characteristics. P. nicotianae was previously reported as the causal agent of heart rot of pineapple in Hawaii, U.S.A. (3) and Guangdong Province of China (1). To our knowledge, this is the first report of P. nicotianae on pineapple in Hainan Province, China. References: (1) J. Z. Chen et al. J. Yunnan Agric. Univ. 8:134, 2003. (2) H. H. Ho. Mycologia 73:705, 1981.(3) K. W. Howard et al. Plant Dis. Rep. 48:848, 1964. (4) X. B. Zheng. Page 81 in: Phytophthora and its Research Technology. China Agricultural Press, Beijing, 1997.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>H F</ForeName><Initials>HF</Initials><AffiliationInfo><Affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>B R</ForeName><Initials>BR</Initials><AffiliationInfo><Affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhan</LastName><ForeName>J X</ForeName><Initials>JX</Initials><AffiliationInfo><Affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pu</LastName><ForeName>X M</ForeName><Initials>XM</Initials><AffiliationInfo><Affiliation>Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30722247</ArticleId><ArticleId IdType="doi">10.1094/PDIS-11-12-1017-PDN</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Shen, H F" sort="Shen, H F" uniqKey="Shen H" first="H F" last="Shen">H F Shen</name></region><name sortKey="Lin, B R" sort="Lin, B R" uniqKey="Lin B" first="B R" last="Lin">B R Lin</name><name sortKey="Pu, X M" sort="Pu, X M" uniqKey="Pu X" first="X M" last="Pu">X M Pu</name><name sortKey="Zhan, J X" sort="Zhan, J X" uniqKey="Zhan J" first="J X" last="Zhan">J X Zhan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001314 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001314 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30722247
|texte= First Report of Pineapple Heart Rot Caused by Phytophthora nicotianae in Hainan Province, China.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30722247" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |